Figure 6
The interaction of S. Typhi flagella derivatives with epithelial cells.
Hep-2 cells were infected with S. Typhi expressing one of three flagellin variants, a non-flagellated mutant (ΔfliC) and an invasion deficient mutant (ΔinvA). (a) Histogram showing the median proportion of recovered intracellular bacteria normalized by the inoculum and averaged over six experiments (*p = 0.0002, for Hj compared to other variants by ANOVA). Error bars show 1 standard deviation from the mean. (b) Histogram showing the number of recovered bacteria during attachment (white columns) and invasion (grey columns) assays averaged over six experiments (*p = 0.0001, for Hj compared to other variants by ANOVA). (c) Transcriptome analysis of infected Hep-2 cells. Figure shows hierarchical clustering of differentially expressed genes in Hep-2 cells infected with S. Typhi expressing one of the three flagellin variants, the non-flagellated mutant (ΔfliC) and the invasion deficient mutant (ΔinvA), compared with uninfected Hep-2 cells. The cut-off for differentially expressed genes was an absolute fold change >2 and FDR corrected p-value of <0.05. Red/green color scale indicates level of gene expression, red indicates increased gene expression and green indicates reduced gene expression.
