Figure 7
The ability of S. Typhi flagella derivatives to induce ruffling in epithelial cells.
(a) SEM images of Hep-2 cells infected with S. Typhi Hd, Hj, and Hz66 flagella derivatives. Images show ruffles induced by S. Typhi inducing cytoskeletal rearrangement, exaggerated by S. Typhi Hj. (b) The role of Rho GTPases during ruffle formation was assessed by using iRNA to block the RAC1, CDC42 and RHOG Rho GTPases. Images prepared by SEM of Hep-2 cells with knock down Rho GTPases or controls infected with S. Typhi. (c) Western blot of cell lysates, confirming the lack of Rho GTPases expression after iRNA. Lanes, 1; untreated cells, 2; reagent control, 3; non-interfering siRNA control, 4; CDC42 siRNA, 5; RAC1 siRNA and 6; RHOG siRNA.
