Figure 5

Replacement of Cys304 with Ala in AM1_1557g2.

(A) CBB-stained C304A after SDS-PAGE (CBB) and in-gel Zn-dependent fluorescence assay (+Zn) showing C304A does not bind BV. (B) Absorption spectra of AM1_1557g2-BV Pfr (broken line) and C304A (solid line).