Figure 7
Fluorescent microscopy.
(A) Transmission and (B) fluorescence images of solutions of (a) free PCB and (b) AM1_1557g2-PCB. PCB was obtained from Frontier Scientific. As a control, 50 µM PCB in Buffer A was used. (C) Change in fluorescent intensity of AM1_1557g2-PCB during (Pr – Pg) photoconversion. Fluorescence from Pr was detected when AM1_1557g2-PCB was irradiated with red light (RL). Red-light irradiation was continuous throughout the experiment. Photoconversion was induced by intermittent green light (GL) irradiation. The fluorescence intensities of AM1_1557g2-PCB and free PCB are plotted against time. (D) Transmission and (E) fluorescence images of solutions of (a) free BV and (b) AM1_1557g2-BV. BV was obtained from Frontier Scientific. As a control, 50 µM BV in Buffer A was used. (F) Change in the fluorescent intensity of AM1_1557g2-BV during (Pfr – Po) photoconversion. Fluorescence from Pfr was detected when AM1_1557g2-BV was irradiated with far-red light (FRL). Far-red light irradiation was continuous throughout the experiment. Photoconversion was induced by intermittent orange-light (OL) irradiation. The fluorescence intensities of AM1_1557g2-BV and free BV are plotted against time.
