Figure 1
From: 3D-DIP-Chip: a microarray-based method to measure genomic DNA damage
Representation of the 3D-DIP-Chip and ChIP-Chip procedure.
(a) Proteins are crosslinked to chromatin (top) or DNA damage is induced (bottom). This DNA or chromatin is extracted, sonicated and split into two samples. (b) IP is carried out on one sample to separate the chromatin bound factor of interest (top) or damaged DNA (bottom). (c) Both samples are purified, amplified by PCR and differentially labelled with red or green fluorophores. (d) The samples are allowed to hybridise to the microarray probes and the resulting intensity values from the scanned image are converted to numerical values. (e) These values may be plotted and processed as required by the investigation.
