Figure 3

Analysis of cell-surface markers and cDNA arrays.
Flow-cytometry data were collected in at least triplicate and at distinct culture periods in order to avoid ongoing culture selection. ORI: original cells not sorted into single cells; DIF: differentiated cells obtained using 10% serum. (a) CD133 expression (no statistically significant difference). (b) CD44 expression. (c) CD24 expression (*P < 0.01). (d) CD56 expression (*P < 0.01). (e) Analysis of cDNA arrays. Fold-changes in gene expression were calculated as the ratio of the signal values of the original cells to the value of each of the clonal cells after duplication.