Figure 5
5-aza stimulates the migration ability of hMSCs through the increase of CXCR2 and CXCR4.
(A-B) After treating hMSCs with 5-aza, CXCR2 and CXCR4 expression was confirmed through (A) real-time qPCR and (B) western blot analysis. (C) After 5-aza treatment, the migration of hMSCs was determined after treatment with the CXCR2 or CXCR4 ligands. (D) Schematic diagrams indicating the locations of the promoter primers. (E) After treating hMSCs with 5-aza, bisulfide conversion and methyl-specific PCR were performed. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Results show one representative experiment out of at least three. Results are shown as mean ± SD.
