Figure 6
From: ZnT2 is a critical mediator of lysosomal-mediated cell death during early mammary gland involution
Expansion of lysosomal Zn pools via ZnT2 increases LCD.
(a) Co-immunofluorescence of Lamp1 and cathepsin B demonstrating that TNFα augmented the presence of lysosomes and cytosolic cathepsin B compared to control siRNA transfected cells and that ZnT2 attenuation abrogated this effect. Scale bars = 10 μm. (b)–(c) Increased cathepsin B activity in lysosome-enriched and cytosolic fractions isolated from TNFα stimulated compared to control siRNA transfected and ZnT2 attenuated cells. The data are the fold change ± SD of three independent experiments; n = 3/treatment, *P < 0.05. (d) Cell number decreased in response to TNFα and ZnT2 attenuation rescued this effect. Data represent cell number as a percentage of control siRNA transfected cells ± SD, n = 3/treatment. Experiment was conducted three independent times. ***P < 0.0001. For all experiments cells were treated with ZnT2 siRNA or control siRNA followed by ± TNFα (15 ng/mL) for 24 h.
