Figure 2
From: Luciferase Reporter Gene Assay on Human 5-HT Receptor: Which Response Element Should Be Chosen?
Activity of 5-HT on different response elements based luciferase assay.
(A) Transfection of either 293T or CHO-K1 cells with CRE-luc and pRL-SV40 plasmids followed by stimulation with 10 μM 5-HT. (B) The maximum S/B resulting from 10 μM 5-HT treatment when using CRE, SRE and NFAT response elements to detect different receptors. (C) Transfection of CHO-K1 cells with the CRE-luc, 5-HT1A receptor and pRL-SV40 plasmids followed by stimulation with 5-HT in the presence of FSK (0.5 μM or 1 μM), luciferase activity was measured. The inhibition rate is expressed as a percentage of the indicated FSK-induced value. (D-F) Detection the activation of 5-HT1A, 5-HT2A and 5-HT7A receptors to different concentrations 5-HT in CRE-luc, NFAT-luc, SRE-luc assay. Luciferase activity was measured. Three independent experiments were performed in triplicate.
