Figure 3

Depletion of 5-HT in wild type mice by inhibition of TPH1 aggravating APAP hepatotoxicity while reloading 5-HT in TPH1-/- mice by 5-HTP alleviating liver injury.

Four groups including sufficient 5-HT (WT and KO+5-HTP groups) and deficient 5-HT (WT+PCPA and KO groups) are recruited to study the role of 5-HT in APAP hepatotoxicity. Mice (n = 6) were given an i.p. injection of acetaminophen (300 mg/kg for 24 hours) and serum and liver tissues were collected. (A), the concentration of 5-HT in the four groups. (B), Liver index of the mice after APAP administration from the four groups. (C, D), ALT and AST levels in serum. (E), Kaplan-Meier survival curve for mice (n = 15) in the four groups during 5 days after a single lethal dose of APAP (600 mg/kg). (F), the HE staining of liver sections from four groups and relative percent of necrosis area. (G), the TUNEL staining of hepatocyte apoptosis and corresponding percentage of positive cells. *P < .0.05 vs WT group, **P < .0.01 vs WT group, ^†P < .0.05 vs TPH1 knockout group, ^††P < .0.01 vs TPH1 knockout group.