Figure 2
From: Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease
Specific autoantigens identified.
(a) Membrane antigens-based ELISA was used to select BD patients who presented relatively higher optical density values of EA.hy926 membrane antigens for further testing. Error bars are shown (mean with SD). (b) Then protein immunoblot was performed to screen the putative binding target. Western blotting of EA.hy926 cell membrane extracts using the sera from BD patients showed a positive band around 36-kDa. Line 1-5 are sera from BD, line 6 is control serum from healthy donor. (c) The amino acid sequence of the positive band was identified by LC-MALDI-TOF-TOF mass spectrometry. BD, Behçet's disease. HC, healthy controls.
