Figure 1 | Scientific Reports

Figure 1

From: Multiple invasions of an infectious retrovirus in cat genomes

Figure 1

Detection of RD-114 proviruses in the genomes of feline cell lines by Southern blotting analyses.

(A) Restriction endonuclease maps of an infectious molecular clone of RD-114 virus, termed pSc3c. Probes for gag, pol and env genes are shown as bars. (B–D) Southern blotting analyses of several feline cell lines. Genomic DNAs were digested with HindIII, SacII, EcoRI or SphI and then subjected to Southern blotting analyses using gag (B), pol (C) and env (D) probes. HindIII-digested fragments corresponding to pSc3c are shown by white arrowheads. Asterisks indicate RD-114 viral env locus detected in common in all cell lines examined.

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