Figure 6
From: Multiple invasions of an infectious retrovirus in cat genomes
Infectivity of RDRS C1.
(A) Differences between RDRS C1 and an infectious molecular clone, pSc3c. Numbers indicate nucleotide locations at pSc3c. Shaded regions are functional domains of pol region predicted by Pfam (PR, aspartyl protease; RT, reverse transcriptase; RH, RNaseH; IN, integrase core domain [Pfam accession number: PF00077, PF00078, PF00075 and PF00665, respectively]). Differences between RDRS C1 and pSc3c are indicated by vertical lines (point mutations) and filled triangle (insertion). RDRS C1 mutants have mutations marked by crosses. (B, C) LacZ marker rescue assay performed using TE671 cells as target cells. Infection with LacZ pseudotype viruses (RD-114 virus, RDRS C1 wild-type and RDRS C1 mutants) were visualized by X-Gal staining (B) and the virus titers were expressed as f.f.u./ml (C). Assays were performed in triplicate and the data are shown as the mean viral titers ± standard errors.
