Figure 4
From: Elabela-Apelin Receptor Signaling Pathway is Functional in Mammalian Systems
Stimulation of tubule-like structure by ELA-APJ signaling.
(A), Representative images illustrating tubule-like formation in HUVECs in the absence or presence of ELA (0.5 μΜ) and in APJ-overexpressing or knocking-down HUVECs. (B), Reverse transcription PCR of human APJ expression of HUVECs infected with APJ or APJ siRNA lentiviral constructs. (C), Dose-dependent increase of the number of branch point by ELA in HUVECs. (D), APJ-dependent effect of ELA on tubule formation. HUVECs infected with empty vector (Control), APJ-overexpressing (APJ O/E) or APJ siRNA vectors were treated without or with ELA (0.1 μΜ). Values are mean + SEM (n = 5); **p < 0.01, compared to empty vector control without ELA.
