Figure 3

Confocal luminescence images of pea aphids given a subcutaneous injection of Cu²⁺ (a, 300 nL, 5 mM in a HEPES buffer solution (20 mM, pH 7.4)), 25 μM RuMAZO (b, 300 nL, 20 mM HEPES), 25 μM RuMAZO and 50 μM Cu²⁺ (c, 300 nL, 20 mM HEPES).

Images were taken after incubation for 6 h. Left: Bright field images. Middle: Dark field images. Rigth: Merged images. λex = 488 nm.