Figure 2
From: A concurrent excitation and inhibition of dopaminergic subpopulations in response to nicotine
Inhibited DA neurons are located in the medial part of the VTA.
(a) Coronal diagram at −3.52 mm from bregma onto which are positioned TH-positive cells labelled in vivo. Red and blue dots respectively represent excited (n = 43) and inhibited (n = 18) cells. Abbreviations: IF, interfascicular nu; IPR, interpedunc nu; ml, medial lemniscus; PN, paranigral nu; Rli, rostral linear nu; RN, red nu; SNc, substantia nigra compacta VTA, ventral tegmental area (scale bar: 0,5 mm). (b) Photomicrographs of two representative TH-positive (left) andneurobiotin-stained (middle) VTA DA neurons, one excited by nicotine (top) and the other inhibited (bottom). A merge micrograph (right) validates the DA phenotype of the recorded neuron. White arrow indicates labelled neuron. (c) Histogram of the labelled cells' anatomical distribution along the medio-lateral axis from the midline, inhibited neurons located at 378 ± 29 μm and excited neurons at 550 ± 32 μm, p < 0.001, Wilcox test.
