Figure 2 | Scientific Reports

Figure 2

From: Truncation and activation of GSK-3β by calpain I: a molecular mechanism links to tau hyperphosphorylation in Alzheimer's disease

Figure 2

Truncation of GSK-3β is positively correlated with site-specific phosphorylation of tau, Tangle Score and Braak State in human brain.

(A) Quantitation of immune-dot-blots (not shown) by densitometry shows hyperphosphorylation of tau at each site in AD brain. Tau phosphorylation at individual phosphorylation sites in the frontal cortical crude extracts from 7 AD and 7 control cases were determined by quantitative immuno-dot-blots and relative signal obtained by densitrometry is shown as mean ± SD (n = 7). **, p < 0.01. (B) Spearman correlation analysis shows a direct correlation of truncation of GSK-3β with tau phosphorylation at various sites. The levels of tau phosphorylation at individual phosphorylation site in AD and control brains same as in panel A (Y-axis) were plotted against the truncation of GSK-3β (ratio of truncated over full-length GSK-3β) (X-axis) from Fig. 1C. (C, D) Spearman correlation analysis shows correlation of truncation of GSK-3β with Tangle Score and Braak Stage. Truncation of GSK-3β from Fig. 1C (Y-axis) were plotted against the Tangle score (C) or Braak Stage (D) (X-axis) from Table S1.

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