Figure 3

Electrostatic surface potential representations of CpsUbiX.
(a) Cartoon view of dodecameric CpsUbiX (green) with bound FMN (purple sticks). Molecular surface representation of CpsUbiX shows a greater accumulation of positively charged residues (Lys45, Lys54, Pro60, Lys65 and Lys83) on the surface at the predicted membrane binding site. (b) Cartoon view of the dodecameric V47S mutant CpsUbiX (cyan) structure without FMN. Molecular surface representation of the V47S mutant CpsUbiX shows markedly reduced net positive surface charge. The molecular surfaces are colored according to electric potential. Blue and red represent positive and negative potentials (units of kT/e from −8 to +8), respectively. The orientation of the surface representation figure is identical to that illustrated in the left panel.