Figure 4
From: Identification of flubendazole as potential anti-neuroblastoma compound in a large cell line screen
Effects of flubendazole on p53 activation and apoptosis in UKF-NB-3 cells.
UKF-NB-3 cells, UKF-NB-3 cells transduced with a lentiviral vector encoding for p53 shRNA (UKF-NB-3p53shRNA) and UKF-NB-3 cells transduced with a control vector (UKF-NB-3scr) were incubated for 24 h with flubendazole 300 nM. (a) Western blots indicating the expression of p53 and its target gene products p21, BAX and PUMA; (b) Western blots indicating caspase 3 (casp. 3) cleavage/activation and cleaved PARP; (c) Western blots indicating siRNA-mediated depletion of p21, BAX, or PUMA in UKF-NB-3 cells (scr. siRNA, non-targeted scrambled siRNA) 24 h post transfection; (d) Influence of the siRNA-mediated depletion of p21, BAX, or PUMA on the viability of UKF-NB-3 cells in the presence of flubendazole. 48 h post transfection with siRNA, cells were transferred into 96-well plates in the absence or presence of serial dilutions of flubendazole. The cell viability was determined after a 5 day incubation period by MTT assay. * P < 0.05 relative to scr. siRNA; # P < 0.05 relative to p21 and BAX siRNA Original Western blots corresponding to Figure 4A, 4B and 4C are displayed in Suppl. Fig. S7.
