Table 1 Quantification of the interaction between the RXR DBD and DNA by ITC. All data were obtained at 25°C (T = 298°K). Values represent the means values of 2–4 independent experiments and errors correspond to one standard deviation. N corresponds to the number of moles of dimer per mole of DNA except for the half-sites where N corresponds to the number of monomer per mole of DNA
From: Structural Basis of Natural Promoter Recognition by the Retinoid X Nuclear Receptor
DNA | Kd nM | N | ΔHobs kcal × mol−1 | ΔSobs cal × mol−1 × deg−1 |
|---|---|---|---|---|
Ramp2-DR1 | 38 ± 15 | 1.1 | −39 ± 3 | −98 |
Gde1-DR1 | 140 ± 20 | 1.3 | −25 ± 3 | −51 |
Gde1SpA-DR1 | 44 ± 5 | 1.3 | −29 ± 1 | −66 |
Nr1d1-DR1 | 40 ± 5 | 1.1 | −38 ± 1 | −92 |
idDR1 | 155 ± 40 | 1.2 | −26 ± 1 | −55 |
350& | ||||
MEp-DR1 | 10 ± 2 | 1.1 | −30 ± 0.3 | −64 |
Half-site 1 | 980 ± 110 | 1.0 | −11 ± 1 | −9 |
Half-site 2 | 820 ± 150 | 1.0 | −6 ± 1 | 6 |
