Figure 6
Ezh2 regulates miR-27a expression during human fibroblasts reprogramming.
(a) Differentially expressed miRNAs in sh-Ezh2 hFibs calculated by Edge R and DESeq programmes. (b) Histogram depicting the number of miRNA regulating indicated pathways in shEzh2 hFibs. (c) qPCR results of indicated miRNA expression levels in shCnt, shEzh2 and Ezh2 OE hFibs. (d) qPCR results of indicated miRNA expression levels in DMSO control and GSK treated hFibs. (e) Expression profile of indicated miRNA in hFibs, OSKM transduced hFibs on day7 and iPSCs. (f) Chromatin IP for Ezh2 and H3K27me3 was performed in hFbs, OSKM transduced hFibs at day7 and iPSC. Two Kilobase upstream sequence of miR27a was amplified from immunoprecipitated material. (g) Schematic representation of miR-27 plasmid overexpression and OSKM transduction. E-cadherin DAB staining was performed 10 days post introduction of reprogramming factors. (h) E-cad DAB staining in H9 hESC, control and miR-27a overexpressing hFibs.
