Figure 2
Putative critical inter-hexameric residues.
(a) The interface between monomer A (pink and blue) and monomer B (yellow and red) is magnified in the insert. Residues with less than 3.5 Å are labelled (Glu47, Gln46, Leu49 and Thr50) and representative inter-residue hydrogen bonds are illustrated (yellow dotted lines). (b) Analytical ultra-centrifugation profile of wildtype (black histogram) and mutant Hcp1Q46AE47A (red histogram) at 2 mg/mL concentration. The molecular weight kD refers to experimental mass.
