Table 2 List of plasmids and strains

From: Extended Loop Region of Hcp1 is Critical for the Assembly and Function of Type VI Secretion System in Burkholderia pseudomallei

Plasmid or Strain

Relevant characteristic(s)a

Source or reference

Plasmid

  

pK18mobsacB

Conjugative, suicide vector, Kmr

48

pFRTT1

pGEM-T contains a tet cassette with FRT sites and tetRA genes, Apr, Tcr

16

pET28a

Protein expression vector with N-terminal His-tag/thrombin/T7-tag and optional C-terminal His-tag

Novagen

pET22b

Protein expression vector with N-terminal His-tag and optional C-terminal His-tag

Novagen

B. pseudomallei

  

KHW

B. pseudomallei wildtype strain

49

Δhcp1

KHW, codon 21–160 of BPSS1498 was removed

This study

ΔclpV

KHW, codon 11–936 of BPSS1502 was removed

This study

KHW hcp1Q46AE47A

KHW, codon 46 and 47 of BPSS1498 substituted with alanine

This study

E. coli

  

S17-1

Donor strain for conjugation

50

  1. aAbbreviations: Apr, ampicillin resistant; Kmr, kanamycin resistant; Tcr, tetracycline resistant, Tmr, trimethoprim resistant.
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