Figure 1
From: Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
Evaluation of Npc1l1 sgRNA:Cas9-mediated modifications of Npc1l1 in pig parthenogenetic embryos.
(a) Schematic diagram of pig Npc1l1 partial protein coding region and the targeting locus of Npc1l1 sgRNA:Cas9. Red arrow indicates the targeting site of Npc1l1 sgRNA:Cas9. (b) Sequencing results of the modified Npc1l1 alleles detected in injected parthenogenetic embryos. Sequences complementary to sgRNA are labeled in red and PAM sequences are in green; mutations, blue, lower case; deletions, (−); insertions, (+). (c) The chromatographs of sequencing modified Npc1/1 alleles in parthenogenetic embryos in which overlapped peaks were observed. Con denotes wild-type sequence. Red arrow indicates the targeting site of Npc1l1 sgRNA:Cas9.
