Figure 4
From: Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
Npc1l1 sgRNA:Cas9-mediated modifications in the ovaries of founder C2-5.
(a) PCR products of the targeted region of Npc1l1 in the ovaries of founder C2-5. (b) Detection of Npc1l1 sgRNA:Cas9-mediated cleavage of Npc1l1 in ovaries. All PCR products from (a) were subjected to T7EN1 cleavage assays. (c) Sequencing results of modified Npc1l1 alleles detected in the ovaries of founder C2-5. PCR products of the targeted region of Npc1l1 were amplified from the ovaries and sequenced. The PAM sequences are highlighted in green; the targeting sequences in red; the mutations are shown in blue, lower case. Deletions, (−); and Insertions, (+). N/N indicates positive colonies out of total sequenced.
