Table 1 Summary of test embryo microinjection of Cas9 mRNA and Npc1l1sgRNA
From: Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
Number of collected ovaries | Number of cultured COCs | Number of mature oocytes | Number of Cas9/sgRNA injection oocytes | Cleavage rate at 48 hr post activation | Blastocyst development rate at 144 hr post activation | |||
|---|---|---|---|---|---|---|---|---|
Injected group | Untreated group | Injected group | Untreated group | Injected group | Untreated group | |||
400 | 810 | 420 | 195 | 225 | 52.8% (103/195)a | 40.4% (91/225)b | 14.4% (28/195)a | 13.3% (30/225)a |
