Table 2 Summary of Npc1l1 alleles from test embryos with Npc1l1 sgRNA:Cas9-mediated modifications

From: Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA

Embryo No.

Target site mutations

#1

overlapped peaks

#2

del 9 bp

#3

del 11 bp

#4

del 7 bp, insert 1 bp

#5

WT

#6

del 2 bp, insert 29 bp

#7

overlapped peaks

#8

overlapped peaks

  1. Del: deletion; insert: insertion.