Table 4 Summary of alleles from founders with Npc1l1 sgRNA:Cas9-mediated modifications
From: Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
Founder No. | Target site mutations | Sequenced colonies |
|---|---|---|
C1-1 | del 24 bp | 8 |
|  | del 9 bp | 6 |
C1-2 | del 16 bp | 7 |
|  | del 9 bp | 8 |
C1-3 | insert 1 bp | 5 |
|  | del 128 bp | 10 |
C1-4 | del 9 bp | 1 |
|  | del 89 bp | 10 |
|  | del 104 bp, insert 270 bp | 2 |
C1-5 | del 59 bp | 10 |
|  | del 9 bp | 6 |
C2-1 | del 42 bp, insert 56 bp | 5 |
|  | del 24 bp | 5 |
|  | del 412 bp | 4 |
C2-2 | del 1 bp, insert 5 bp | 4 |
|  | del 9 bp | 10 |
C2-3 | del 24 bp, insert 7 bp | 3 |
|  | del 12 bp | 2 |
|  | del 9 bp | 9 |
C2-4 | del 9 bp | 12 |
|  | del 6 bp, insert 535 bp | 1 |
C2-5 | del 13 bp | 6 |
|  | insert 1 bp | 2 |
|  | del 9 bp | 3 |
|  | del 24 bp | 1 |
C2-6 | del 28 bp | 3 |
|  | del 24 bp | 2 |
|  | del 9 bp, insert 2 bp | 7 |
|  | del 12 bp, insert 509 bp | 1 |
