Table 5 Summary of Npc1l1 alleles from different tissues with Npc1l1 sgRNA:Cas9-mediated modifications

From: Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA

Tissues

Target site mutations

Sequenced colonies

ear

insert 1 bp

2

 

del 13 bp

2

 

del 24 bp

3

 

del 9 bp

5

heart

insert 1 bp

6

 

del 13 bp

1

 

del 24 bp

1

 

del 9 bp

1

liver

insert 1 bp

5

 

del 13 bp

2

 

del 24 bp

1

 

del 9 bp

3

  1. Del: deletion; insert: insertion.
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