Figure 5
Decrease in protein levels of hepatic transcription factors and induction of Tnfα expression in Cic-L-/- liver at P10.
(a) Measurement of hepatic BA concentration in 10 day-old WT and Cic-L-/- mice (n = 5 per each genotype). All error bars show s.e.m. (b) qRT-PCR analysis for levels of Shp mRNA in livers from 10 day-old WT and Cic-L-/- mice (n = 3 per each genotype). Error bar shows s.e.m. (c) Western blot image showing the decrease in FOXA2, C/EBPβ and RXRα protein levels in Cic-L-/- liver at P10. The right panel is a bar graph for quantitative analysis on levels of FOXA2, C/EBPβ and RXRα based on Western blot image. *P<0.05 and #P = 0.0587. All error bars show s.e.m. (d) qRT-PCR analysis for levels of eighteen drug metabolism genes in livers from 10 day-old WT and Cic-L-/- mice (n = 3 per each genotype). *P<0.05 and ***P<0.001. All error bars show s.e.m. (e) qRT-PCR analysis for levels of membrane transporter genes in livers from 10 day-old WT and Cic-L-/- mice (n = 3 per each genotype). *P<0.05 and **P<0.01. All error bars show s.e.m. (f) qRT-PCR analysis for levels of Il-1β, Il-6, and Tnfα using liver total RNAs prepared from 10 day-old WT and Cic-L-/- mice (n = 4 per each genotype). *P<0.05. All error bars show s.e.m.
