Figure 2
From: A preliminary exploration on DNA methylation of transgene across generations in transgenic rats
Quantitation of methylation status of transgene in the liver and kidney.
Totally 23 CpG dinucleotides of 5 rats in three generations were studied in these two tissues. Each circle represents the methylation status on one CpG site of one DNA strand. The rate of DNA methylation of each CpG on each strand was quantitated from the peaks of Sanger bisulfite sequencing. The data of methylation status were shown on two strands (Watson and Crick) separately. Totally about 250 ng of genomic DNA was input into each experiment, which was equivalent to about 38,000 pooled DNA molecules. The average percentage of methylation on each CpG dinucleotide was measured by Sanger sequencing. In each circle, the blue color represents the percentage of unmethylated CpG at this site; the yellow color represents the percentage of methylated CpG at this site.
