Figure 2
Effects of inhibitors of protein kinase A (PKA) and protein phosphatase (PP) on sirtinol-induced inhibition of HNE activity.
Human neutrophils were preincubated for 5 min with (A) H89 (3 μM), (B) okadaic acid (1 nM), or (C) calyculin A (10 nM) before the addition of sirtinol (0.3–20 μM) or PGE1 (3 μM). Cells were induced by fMLF/CB for 10 min and HNE activity was measured spectrophotometrically at 405 nm. All data are expressed as the mean ± S.E.M. *** p < 0.001 compared with the corresponding control.
