Figure 1
From: Prion Amplification and Hierarchical Bayesian Modeling Refine Detection of Prion Infection
Correlation between IHC score and sPMCA score of each sample.
(a) Sample scores by IHC and sPMCA were compared by linear regression to evaluate correlation between the two tests. Samples found positive by both tests are considered in “Agreement” (black circles). Samples found negative by IHC but positive by sPMCA are considered in “Disagreement (grey triangles). Each data point represents the mean of all replicates per animal. (b) Representative IHC of a positive obex sample. Scale bars, 100 μm. (c) Representative western blots: Lane 1, undigested NBH; lanes 2–3, negative elk obex from ND and MT after 6 rounds of sPMCA. Lanes 4–5, RMNP elk obex featured in (b) after 3 and after 6 sPMCA rounds (lanes 6 & 7).
