Figure 3
From: Prion Amplification and Hierarchical Bayesian Modeling Refine Detection of Prion Infection
Specificity and sensitivity estimates of sPMCA and IHC.
(a) Specificity estimates of sPMCA by amplification round. Three sample groups are presented: Known samples are positive controls used in previous studies and presented as raw data; Trusted samples are those which are verified by IHC or are from the 2011 sampling; and Unknown samples are all remaining samples which may include contamination-related false positives. (b) Overall sPMCA specificity estimates for Trusted and Unknown samples. The overall specificity of sPMCA is estimated at 93.9% (CI 90.1–96.9%) for Trusted samples and 63.4% (CI 52.6–74.1%) for Unknown samples. (c) Sensitivity of sPMCA by round. Sensitivity increases with each amplification round to 94.68% (CI 83.12–99.84%) by round 6. (d) Overall sensitivity estimates for IHC and sPMCA. Sensitivity estimates for IHC by individual tissue (obex (76.56%, CI 57.00–91.46%) and RPLN (90.06%, CI 74.13–98.70%)) and combined 98.99% (CI 90.01–100%); one replicate of sPMCA after 6 rounds (94.68%, CI 83.12–99.84%); and two replicates of sPMCA (99.51%, CI 97.15–100%).
