Figure 1 | Scientific Reports

Figure 1

From: Aurora B-dependent phosphorylation of Ataxin-10 promotes the interaction between Ataxin-10 and Plk1 in cytokinesis

Figure 1

Interaction between Aurora B and Ataxin-10.

(a) Co-IP between endogenous Ataxin-10 and Aurora B. Aurora B immunoprecipitates were blotted with anti-Ataxin-10 antibodies. IgG immunoprecipitates were negative controls. Two anti-Aurora B antibodies were used for IB: Bethyl (A300–431A) and Santa Cruz (SC-25426). (b and c) Co-IP between FLAG-Ataxin-10 and HA-Aurora B. HeLa cells were transfected with vector or FLAG-Ataxin-10, HA-Aurora B constructs, treated or untreated with Noc. (d and e) Bacterially expressed GST-Aurora B was incubated with lysates from HeLa cells transfected with FLAG-Ataxin-10 (d), or GST-Ataxin-10 incubated with HA-Aurora B (e). (f) GST-Ataxin-10 and His-Aurora B were both bacterially expressed, incubated together and were subject to pull-down assays. Uncropped images of blots were shown in supplemental Figure S1.

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