Figure 4 | Scientific Reports

Figure 4

From: Aurora B-dependent phosphorylation of Ataxin-10 promotes the interaction between Ataxin-10 and Plk1 in cytokinesis

Figure 4

Association between Ataxin-10 and Plk1 is regulated by Aurora B-dependent phosphorylation.

(a) HeLa cells were transfected with vector or FLAG-Ataxin-10-WT, or FLAG-Ataxin-10-S12A and HA-Plk1 constructs. (b) GST-Ataxin-10 and GST-Ataxin-10-S12A were incubated with His-Plk1 and were subject to pull-down assays. (c) Recombinant GST-tagged wild-type Ataxin-10 or the S12A mutant were incubated with GST-Plk1 and subject to IVK assay, then blotted with antibodies indicated. Numbers on the bottom of the Western blot indicate quantification of protein levels. Uncropped images of blots were shown in supplemental Figure S1.

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