Figure 5
From: Piezo1 forms mechanosensitive ion channels in the human MCF-7 breast cancer cell line
Single channel conductance properties of hPiezo1 expressed in the MCF-10A cell line.
(5a).: Original registration from untransfected MCF-10A cell (configuration shown schematically at top). (5b).: same as 5A, but cell was transfected with biscistronic pIRES2 containing hPiezo1 and eGFP as inserts. (5c).: Average I/V relation for single IΛ inward currents carried by 153 mmole/L K+. Line represents a linear regression through the data (N = 5), dotted line represents average IΛ recorded from HEK-293 cells under identical conditions. (5d).: left: Quantitative RT-PCR assessing the relative amount of hPiezo1 mRNA transcripts of MCF-10AWT cells compared to MCF-7WT. Mean values ± SEM are shown (N = 3). Right: the fold increase in the number of hPiezo1 mRNA transcripts upon transient transfection of MCF-10A with the pIRES2 construct. Mean values ± SEM are shown (N = 3).
