Figure 1
From: The molecular architecture of dihydropyrindine receptor/L-type Ca2+ channel complex
Biochemical characterization and electron microscopy of purified DHPR.
(a) Left: Electrophoresis analysis of DHPR. Lane 1, molecular markers; lane 2, DHPR in reducing conditions; lane 3, DHPR in non-reducing conditions; lane 4, DHPR in native conditions. All subunits of DHPR, including the α1 (176 kDa), α2 (147 kDa), β (56 kDa), γ (34 kDa) and δ (20 kDa), exist in the purified DHPR as indicated. Right: Western blotting analysis. lane 1, anti-α1 in reducing conditions; lane 2, anti-α2 in reducing conditions; lane 3, anti-α2 in non-reducing conditions; lane 4, anti-β in reducing conditions. (b) Electron microscopic picture of negatively-stained DHPR sample. (c) Cryo-EM picture of DHPR particles embedded in vitreous ice. Individual DHPR particles are clearly identified and boxed.
