Figure 1
From: Inhibitors of glutamate release from breast cancer cells; new targets for cancer-induced bone-pain
High-throughput screening of 29,586 compounds for inhibitors of glutamate release by MDA-MB-231 cells.
The level of glutamate in the cell medium was measured by Amplex Red reagent 48 hours post inoculation. Fluorescence was read every 90 seconds for 15 minutes. The rate change of fluorescent signal versus time was used as indicator of inhibitory potency. The result was expressed as the ratio of rate change between a testing compound (10 μM) and the positive control, SSZ (200 μM). The X and Y-axis represent the ratio of rate change of replicate 1 (R1) and replicate 2 (R2). Among 500 positive hits, 320 (enclosed in box) showed similar to more potent glutamate release inhibition potency as SSZ and were selected for secondary screening.
