Figure 1
From: How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila
The Drosophila ARousal Tracking (DART) system.
(a). Left: DART software records movies via a webcam while simultaneously controlling motor stimuli via a digital to analog converter (DAC). The multiple motors (represented by red dashed circles) are connected to the DAC via a simple circuit board. Middle: Six platforms are positioned in a 3 × 2 arrangement of 17 flies per platform, with two motors per platform. Right: Each platform has two motors glued underneath. The platform has four pedestals (clear circles) that slot into a larger base. (b). Movement traces for 17 flies over four days (yellow background) and nights (grey) outputted from DART. X-axis is time of day and y-axis is horizontal displacement in the tube for each fly. The different colors (blue, red, green purple) are assigned so that the individual traces are easier to distinguish. Graphical user interface supervision allows selection of unusable flies: black traces (flies 8 and 11) have been marked for removal, as flies appeared to have died. Artwork: Benjamin Kottler and Bruno van Swinderen.
