Figure 3
From: How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila
Hourly arousal probing reveals significant differences between white strains.
(a). Average sleep profiles over several days (yellow) and nights (grey) for w1118 (blue) and w2202 (red). Minutes of sleep per hour were determined by absolute location data, based on a 3 mm movement threshold as in Fig. 2b. All flies were stimulated hourly (by vibrations, indicated by the black lines above the graph). N = 47 for w1118 and N = 50 for w2202. (b). Average speed of w1118 (blue) and w2202 (red) for the same set of flies, over the same time period as in a. The responses to the vibrations (black lines above the graph) are evident as spikes in the speed. Data are averaged for all flies, not only immobile ones. (c). Average speed data for day and night for the same set of flies. The vertical dashed lines indicate hourly vibrations testing for behavioral responsiveness. (d). Characterization of behavioral responsiveness curve. Average speed (purple) (±SEM, light blue) is shown for a wild-type strain, with four metrics derived from a best fit (yellow trace) of the average response. Fitted parameters were: the average speed 1 min before the stimulus (or the baseline speed (VPre-Stimuli), the average speed 1 min after the stimulus (Post-Stimuli Speed), the amplitude of the response (VAmplitude) and the time constant of the response (τInactivation). Green dashed line indicates timing of vibration stimulus (T = 0). Flies analyzed for responsiveness profiles were not necessarily immobile. (e). Average response (±SEM) for w1118, for day (yellow) and night (grey). (f). Average response (±SEM) for w2202, for day (yellow) and night (grey). (g). Best fitting response parameters (as outlined in d) for w1118 and w2202 during the day (yellow) and night (grey). N = 2256 responses for w1118 and N = 2400 responses for w2202, divided equally among 47 and 50 flies, respectively, for day and night. (h). Scatterplot of Pre-Stimuli speed versus Post-Stimuli speed, for individual day (yellow dots) and night (grey dots) events in w1118. A linear regression of the data (±SEE) is shown, with associated slope and correlation indicated. (i). Scatterplot of Pre-Stimuli speed versus Post-Stimuli speed, for individual day (yellow dots) and night (grey dots) events in w2202. A regression of the data (±SEE) is shown, with associated slope and correlation indicated.
