Figure 4
From: How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila
Sleep intensity differs between the white strains.
(a). Responses were binned according to the duration of prior immobility. Vibration stimuli were delivered once an hour over several days and nights (green arrow). Prior immobility epochs were automatically classified into 5 min bins, as determined by the first retroactive detection of any movement, based on a 3 mm threshold. Four examples are shown, where flies had been immobile for different lengths of time prior to the stimulus (red, blue and purple lines) and one fly that was moving immediately before the stimulus (gold line). Only immobile flies were included in the subsequent sleep intensity analyses, which summed locomotion responses 1 min after the stimulus (Action time zone). The y-axis represents horizontal fly movement in the tubes. Note that time before and after the stimulus is not on the same scale in the schema. (b). Sleep intensity in w1118. Left panel: frequency count for every 5 min immobility bin, for day (yellow) and night (black). Middle panel: the proportion of flies responding (±SEM) for each immobility bin, for day and night. Right panel: the average amplitude of the response for each immobility bin (average speed ± SEM), for day and night. (c). The same sleep intensity calculations were performed for w2202 N = 47 for w1118 and N = 50 for w2202. See Tables S1–4 for corresponding statistics.
