Figure 5
From: How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila
Sleep rebound following a random stimulation protocol.
(a). Random patterns of vibration stimuli (set at 1.2 g) were delivered for 12 hours of night. (b). Average hourly sleep duration (±SEM) in w2202 before (blue), during (purple box) and after (magenta and green) the random stimulation. Yellow background indicates day, grey is night. (c). Traditional sleep duration metrics (±SEM) for the data from b, in the same color scheme. *, p < 0.05; **, P < 0.01, by pair-wise 2-tailed t-test. (d). Average speed (±SEM) for the same flies as in b. (e). Average daytime and nighttime behavioral responsiveness (±SEM) for baseline and two successive days following the random stimulation. Velocity data (Vamplitude) are summarized in the panel on the right, for the same three days. **, P < 0.01, by pair-wise 2-tailed t-test. Yellow plots represent daytime responses whereas grey plots are nighttime responses; blue surrounding indicates the day before the random stimulation, magenta surrounding is one day after the random stimulation and green surrounding is two days after random stimulation. N = 17 flies.
