Figure 2

The location of TNF-α and PRDX6 following cSCI.
(A) Localisation of TNF-α and PRDX6 in the spinal cord. DAPI (blue) stains the nucleus, TNF-α (red), PRDX6 (green) and merged images of spinal cord neurons and neuroglial cells after cSCI. TNF-α and PRDX6 are co-localised in neurons and neuroglial cells of the anterior horn of the spinal cord. Magnitude:40 × 10 μm (B) Immunohistochemistry shows that TNF-α was located in the cytoplasm of neurons of anterior horn in spinal cord. (C) The number of TNF-α-positive cells was significantly increased at 24 h and 3 dpo after spinal cord injury. Magnitude:40 × 10 μm. Values are expressed in mean ± SED * p < 0.05 vs. sham group.