Figure 4 | Scientific Reports

Figure 4

From: Lentivirus-mediated Inhibition of Tumour Necrosis Factor-α improves motor function associated with PRDX6 in spinal cord contusion rats

Figure 4

Preparation of TNF-α-RNAi-LV recombinants.

(A) The schematic of TNF-α-RNAi-LV. Enhanced red fluorescent protein (mcherryFP) as a reporter gene was inserted in the plasmid. The framework also contains the antibiotic ampicillin and pUC Ori promoters for vector expression. (B) qRT-PCR shows effective interference of fragment for TNF-α inhibition, the expression of TNF-α were reduced. (C) Fluorescent image of TNF-α-RNAi-LV transfected into 293T cells.

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