Figure 3
Inhibition of PKR decreases BACE1 levels and Aβ production in mice hippocampus after LPS systemic challenge.
Immunoblot analysis (A) and protein levels of mature BACE1 (B) and APP (C) and quantification of Aβ1–42 by ELISA (D) in WT and PKR-/- mice treated with saline or LPS. LPS induces BACE1 maturation and Aβ production without altering APP levels in WT mice. PKR inhibition prevents BACE1 maturation and Aβ production after LPS treatment. Transcriptional activity on BACE1 in hippocampus assessed with qRT-PCR and normalized to GAPDH mRNA levels (E). Immunoblots (A) have been cropped in this figure. Full length version of BACE1 and APP blots are available in the supplementary Figure 5. WT [n = 4], PKR-/- [n = 4], **p < 0.01.
