Figure 4
Activation of transcription factor STAT3 after LPS systemic challenge is down-regulated by PKR but not translation initiation factor eIF2α.
Immunoblot analysis (A) and corresponding quantification of peIF2αSer51/eIF2α (B) and pSTAT3Tyr705/STAT3 (C) ratios in hippocampus of WT and PKR-/- mice treated with saline or LPS. LPS induces STAT3 but not eIF2α activation in WT mice. PKR inhibition prevents STAT3 activation. Cropped immunoblots are presented in this figure. Full length immunoblots of eIF2α (phosphorylated and total forms) and STAT3 (phosphorylated and total forms) are available in the supplementary Figure 6. WT [n = 4], *p < 0.05, **p < 0.01.
