Figure 3
The hsa-miR-128-3p suppressed endogenous CYP2C9 expression in HepaRG cells.
Differentiated HepaRG cells were transiently transfected with 25 nmol/L or 50 nmol/L hsa-miR-128-3p mimic, CYP2C9-specific siRNA, or miRNA negative control, respectively and harvested 48 hours after transfection. Each assay was done on at least 3 independent transfection experiments. NC, miRNA negative control; * P < 0.05; ** P < 0.001. (a) Unregulated expression of hsa-miR-128-3p. Data were shown as relative hsa-miR-128-3p levels versus U6. (b) Down-regulated expression of CYP2C9 mRNA by hsa-miR-128-3p or CYP2C9-specific siRNA. Data are shown as relative CYP2C9 mRNA levels versus GAPDH reference. (c) and (d) Down-regulated CYP2C9 protein levels by hsa-miR-128-3p or CYP2C9-specific siRNA.
