Figure 3
Cellular effects of KRAS mutants.
(a) MCF10A clones expressing the indicated KRAS mutations at low (white letters) and high (red letters) levels were grown in regular medium to 50% confluency. Representative phase contrast micrographs (original magnification, 10×) are shown. Insets show 2-fold magnified details of the corresponding photographs, highlighting cells with lamellopodia formation (arrows). (b) Cell viability and proliferation of MCF10A clones expressing different KRAS mutations and cultured with and without EGF for four days, relative to the mean viability and proliferation of EV-transduced cells and cells expressing WT KRAS (dotted line). Results of three independent experiments performed in triplicate are shown (mean ± SEM). P values were calculated using an unpaired t-test. (c) Anchorage-independent growth in soft agar of MCF10A cells expressing different KRAS mutations at low and high levels, relative to colony formation of EV-transduced cells. Shown are colony numbers after six weeks (two to three independent experiments performed in triplicate, mean ± SEM). P values were calculated using an unpaired t-test. (d) Migration and wound healing of MCF10A cells expressing different KRAS mutations at low and high levels. Shown is the percentage of wound closure 24 hours after scratching a confluent monolayer (three independent experiments, mean ± SEM). *, P < 0.05; **, P < 0.01; ***, P < 0.0001; ns, not significant.
