Table 1 Effects of diverse KRAS mutations expressed at physiological levels in MCF10A cells
| Â | WT | G12C | G12D | G12V | G13C | G13D | A18D | Q61H | K117N |
|---|---|---|---|---|---|---|---|---|---|
KRAS-GTP | − | + | − | + | + | − | − | ++ | ++ |
Pathway activation # | Â | Â | Â | Â | Â | Â | Â | Â | Â |
• EGFR | − | − | − | − | ++ | +++ | − | − | − |
• p-p53 | − | − | − | − | − | +++ | − | − | − |
Morphology | − | − | − | − | − | − | − | − | − |
EGF-independent proliferation | − | − | +++ | ++ | − | +++ | − | − | ++ |
Anchorage-independent growth | − | + | − | + | − | − | − | ++ | +++ |
Migration | − | − | − | − | − | − | − | − | − |
Transcriptional changes in G13D versus G12D | − | ND | Luminal BC | ND | ND | Basal BC | ND | ND | ND |
| Â | Â | Â | Â | Â | Â | Cytokine signalling up | Â | Â | Â |
| Â | Â | Â | Â | Â | Â | p53 signalling up | Â | Â | Â |
